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The side-chain guanidyl group of Arg454 interacts with the carboxylate moiety of glycine to orient the substrate for attack on the acyl-phosphate intermediate (Herrera et al., Unlike other organisms, some plants synthesize glutathione analogs in which -alanine, serine, or glutamate replaces glycine in the tripeptide (Figure 4A)

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Dramatic overnight changes are unlikely, with healing proceeding incrementally

Consequently, the therapeutic promise for DCM lies not in generic copper restriction but in strategies capable of rectifying this specific copper mislocalization