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Hepatic tissues were ground into powders with liquid nitrogen and hepatic cells were homogenized in RIPA lysis buffer (1% leagene PMSF, 1% protease and Phosphatase Inhibitor Cocktail)

In mice with age-related cognitive impairment, GHK demonstrated a partial improvement in cognitive function through anti-inflammatory and epigenetic mechanisms

In patients with normal kidney function, it was strongly and significantly associated with the risk of venous thromboembolism (Brodin 2012)