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Biosynthesis and occurrence [edit] Glutathione biosynthesis involves two adenosine triphosphate-dependent steps: First, -glutamylcysteine is synthesized from L-glutamate and L-cysteine

This result highlights that rather than synthesizing GSH, cysteine has other downstream fates of greater importance, such as protein translation or cysteine polysulfidation 50

However, these findings also indicate that the effects of PYC may be context-dependent and may exhibit pro-oxidant properties under certain conditions, warranting further evaluation (Youm and Kim, 2003)

Identification of a putative antioxidant response element in the 5-flanking region of the human -glutamylcysteine synthetase heavy subunit gene

DNA extractions were performed on 500 mg of feces per sample using the FastDNA SPIN Kit for Soil (MP Biomedicals, Solon, CA, USA)