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Spotting Assay Media was made up on the day prior to analysis and 8 mM DTT or 1 mM hydrogen peroxide with or without 5 mM carnitine was added where appropriate as previously described ( 600nm of 1 and five sequential ten times dilutions were made in a microtiter plate

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To assess this issue, we crossed Stard7 Lox/lox or Stard7 IEC mice with the Apc +/Min strain which spontaneously develops intestinal adenomas due to constitutive Wnt signaling (Su et al, 1992)