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We report a novel retrosynthetic strategy for Ohauamine C that employs an unprecedented early-stage esterification to preorganize the molecular conformation, enabling efficient macrocyclization

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Their work revealed Km values of approximately 0.0528 mM and 0.0565 mM for two strains, with corresponding Vmax values of 55.24 U/mL and 71.13 U/mL, indicating strong substrate affinity and catalytic efficiency