J Biol Chem 281 : 1576315773
the freeze-drying process comprises the following steps, preparing a liquid and filling at the temperature of 20-30 , putting a sample into a freeze dryer, quickly reducing the temperature of a plate layer to-40 , preserving heat for 1h, when the temperature of a partition plate is-10 +/-2 , preserving heat for 1h, quickly reducing the temperature of the plate layer to-40 within 1h, preserving heat for 3h, transferring to a cold trap at-50 , vacuumizing to below 10Pa, firstly preserving the temperature of the plate layer at-5 for 3h, then heating the plate layer to 10 within 3h, when a product is at 0 , starting to increase the temperature of the plate layer to 40 , and when the temperature of the product is increased to 30 , preserving heat for 3 h
Survival was evaluated from the time of surgery, with death as the end point
During sleep, your body repairs cellular damage and replenishes antioxidant stores
Abbreviations RGCs: retinal ganglion cells GFAP: glial fibrillary acidic protein NOS: nitric oxide synthase NO: nitric oxide iNOS: inducible NOS MTC: methylcellulose Ub: ubiquitin MDA: malondialdehyde IOP: intraocular pressure References Cedrone C, Mancino R, Cerulli A, Cesareo M, Nucci C
