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In addition to this issue, the clinical translation of engineered live bacteria also needs to address key problems such as the genetic stability of strains, long-term assessment of biological safety, and risk control for immunodeficient populations, and establish a complete regulatory system
Analysis of genetic null alleles of fbf-1 and fbf-2 identified an additional role for fbf in regulating the balance between proliferation and differentiation of GSCs ( fbf-1 and fbf- 2 resulted in a failure to initiate oogenesis, resulting in masculinization of the germline ( glp-1(null) mutants as the progenitor zone did not become fully depleted until later in larval development, and depletion did not fully occur at higher temperatures ( fbf-1 or fbf-2 alone resulted in relatively wild-type germlines, with the presence of a distal mitotic GSC pool and developing oocytes, suggesting that similar to their role in sex determination, fbf-1 and fbf-2 function redundantly to regulate the GSC proliferation vs
