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Plant genomic DNA that had been extracted was measured with a NanoDrop 1000 spectrophotometer (Thermo Scientific) followed by dilution to a 50ng/l concentration

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BAL fluid, cell lysates, or apical culture supernatants were incubated with glutathione peroxidase (Sigma-Aldrich, G6137), cumene hydroperoxide (Sigma-Aldrich, 247502), glutathione reductase (Sigma-Aldrich, G9297), and NADPH (Sigma-Aldrich, N1630) for 30 minutes at 25C

At a second consultation, I will confirm that the procedure is appropriate for them and that they are fully aware of the surgical undertaking and the likely downside and recovery time required