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Vitamin C supplementation alleviates hypercortisolemia caused by chronic stress

Alternatively, glutathione can be quantified after reduction of GSSG in the sample with dithiotreitol (DTT) and subsequent derivatization of GSH with mbrB or mchlB via a reverse-phase high-performance liquid chromatography (HPLC) system equipped with a fluorescent or UV detector, or spectrophotometrically after a GR-mediated glutathione recycling assay (total glutathione) from cell organelles after isolation or fractionation

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For immunochemistry, the following primary antibody was used: Anti-GPX4 (1:400, ab125066, Abcam, USA), Anti-ACSL4 (1:800, ab155282, Abcam, USA)

[,,] Mania in bipolar disorder is linked to mitochondrial hyperactivity, while the depressive and euthymic phases are related to mitochondrial underfunction