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Subsequently, treatments with eye drop preparations containing hylauronic acid with or without GSH-C4 (25 L/well and 50 L/well of eye drop products) was initiated both in the absence and presence of Lipopolysaccharide (LPS) (1 g/mL) or hydrogen peroxide (H 2 O 2 ) (25 M) added to induce stress conditions

In addition, GSH levels are affected by nutritional status and hormonal/stress levels, exhibit developmental and diurnal variations, and are affected by certain physiological states, including pregnancy and exercise 38,39,40,41,42,43,44,45

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Genes within this module include PAEP , glutathione peroxidase 3 ( GPX3 ), C-X-C motif chemokine ligand 14 ( CXCL14 ) and dipeptidyl peptidase 4 ( DPP4 ), all previously pinpointed as pivotal genes during the WOI [26, 27]