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After 14 days, cells were trypsinized and replated with N2B27 medium on poly-D-lysine/laminin-coated 16-18mm coverslips for terminal differentiation in the presence of BDNF (R&D Systems, 10 ng/ml)

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c-MET receptor is also associated with GRB2-associated binding protein 1 (GAB1) phosphorylation and provides extra binding sites for other adaptor proteins

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