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The purpose should be very clear and simple, such as you don't want your family or children to become passive smokers because of you

Plasmid construction and tobacco transgenic plant Total RNA was purified from the tobacco leaf and cDNA was obtained with the First Strand cDNA Synthesis kit (Qiagen, Hilden, Germany)

These include heated tobacco products (also known as heat-not-burn products), electronic cigarettes (also known as electronic nicotine delivery systems or ENDS), and oral nicotine pouches

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