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The work was supported by a grant from the Czech Science Foundation (23-04754X) to D.B, and funded by the European Union under Horizon Europe program HORIZON-WIDERA-2023-ACCESS-04, Grant Agreement No

The design principle of enzyme-cleavable linkers is to take advantage of the high expression of specific enzymes inside tumor cells, so that the linker is recognized and cleaved by certain proteases after entering the target cell

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Within laboratory research systems, mechanistic evaluation may include receptor-binding analysis, observation of intracellular signaling cascades, assessment of gene expression responses, and mapping of downstream regulatory pathways associated with IGF-mediated signaling under controlled in vitro conditions