Conflicting conclusions about the existence of stable, catalytically active GSTP1 monomers have been reported, but the preponderance of the data indicate that catalytically active GSTP1 monomers are likely not present in cells (for a summary, see Fabrini et al
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For cell immunofluorescence staining, a similar procedure was performed, except the cell samples were fixed with 4% paraformaldehyde (PFA) (Beyotime, #P0099) for 10 min and permeabilized with 0.5% Triton X-100 (Beyotime, #P0096) for 10 min at room temperature
Li D, Chen X, Dai W, Jin Q, Wang D, Ji J, Tang B Z
Pharmacol Rev 56(3):387437 Singh S, Swarnkar S, Goswami P, Nath C (2011) Astrocytes and microglia: responses to neuropathological conditions