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This reagent reacts with the thiol groups of GSH which convert 2-nitro-5-thiobenzoate (NTB ) to NTB 2 , thereby producing a yellow color, which can subsequently be quantified ( Glutathione Peroxidase GPx is measured by adding glutathione reductase (GR) to the sample after which nicotinamide adenine dinucleotide phosphate (NADPH) is transformed to a reduced state, allowing a decrease in absorbance to be subsequently measured (30, 32)

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