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Campochiaros team examined the safety, tolerability and visual function effects of oral NAC

Finding the optimal buffer composition: high salt concentration (for example, optimal purity can typically be achieved with buffer containing 300 mM NaCl at pH 8.0 for some proteins), pH, as well as addition of the right type of detergents at the right concentration (this is particularly important for membrane proteins or the proteins prone to aggregate)

A blood sample is collected from the mother, serum is separated, and the marker levels are analyzed using immunoassay techniques

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