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Semen cryopreservation, drug treatments, and experimental group settings Before semen cryopreservation, six semen samples were individually split into 4 equals and diluted to a final concentration of 6.0 10 7 /ml spermatozoa with the basic extender supplemented with 0 g/ml SeNPs and 0 mM GSH (negative control group, NC), 2 g/ml SeNPs (SeNPs group) (27), 4 mM GSH (GSH group) (50) and 4 mM GSH plus 2 g/ml SeNPs (GSH + SeNPs group)

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Two P-Thrs in SF3B1 are required for interaction with SNIP1s FHA domain Given that SNIP1 harbors a candidate P-Thr-binding FHA domain at its C-terminus, we tested whether this domain is necessary for interaction with P-SF3B1 (Fig

While not directly measuring glutathione-pathway genetics, understanding individual metabolic predisposition and inflammatory tendency can inform whether antioxidant support aligns with overall aging and metabolic goals

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