Subcutaneous injection of PR65 in iron-depleted hepcidin knockout mice blocks iron overload in the liver, reduces iron levels in the heart, and causes iron retention in the spleen and duodenum
Acknowledgements This work was supported by the National Research, Development and Innovation Office of Hungary (2020-1.1.2-PIACI-KFI-2020-00039, SNN 151010 and STARTING 152137) and PharmaLab (RRF-2.3.1-21-2022-00015) projects
Membranes were probed using antibodies to FoxO1 (1:2500, 18592-1AP, Proteintech, Chicago, IL, USA), SOD2 (1:6000, 66474-1lg, Proteintech, Chicago, IL, USA), NOX4 (1:600, 14347-1-AP, Proteintech, Chicago, IL, USA), and -actin (1:2000, AM10218-400, Abgent) as the primary antibodies
Preclinical findings do not predict human outcomes, and no safety or efficacy claims are made
TABLE 3 Role of non-CYP450 proteins involved in drug metabolism/transport and impact of cigarette smoking on their activity