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Even though smoking rates in the US have decreased over time, cigarettes still remain the leading cause of preventable death in the US, according to the Centers for Disease Control and Prevention

Following the transfer, membranes were blocked with Superblock (Thermo Fisher Scientific) for 1 h at room temperature and then incubated overnight at 4C with the following primary antibodies: phospho-c-Jun N-terminal kinase (p-JNK), JNK (Cell Signaling Technology, MA, United States), hepatocyte nuclear factor 4 alpha (HNF4) (Abcam), CYP2E1, CYP1A2, CYP3A11, and -actin (Santa Cruz Biotechnology, CA, United States)

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