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In the LTA, pupils were classified into unobserved (latent) groups characterized by whether they changed their smoking/vaping injunctive and descriptive norms (favorable towards smoking or against smoking) between baseline and follow-up

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To ensure RNA amplification accuracy on the RT-qPCR platform, each sample underwent DNase treatment with Qiagens RNase-free DNase Set according to the manufacturers instructions

In the original New England Journal of Medicine study, it was a simple 2015 era vape pen with freebase nicotine that demolished nicotine replacement therapy