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Future applications of GLP-1-RA may extend beyond current differentiation strategies, potentially enabling targeted tissue-specific modulation through biomaterial integration, gene editing, or combination therapies

Cell viability assay Cell viability of CuCl 2 (Merck) or CuSO 4 (Merck) -treated HepG2 and HepG2 ATP7B-KO cells was measurement by cell counting assay (CCK-8, Sigma-Aldrich)

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The study was conducted at the Center for Social and Affective Neuroscience at Linkping University in Linkping, Sweden from October 2020 to November 2023 and approved by the Swedish Ethical Review Authority (Dnr 2020-57043), the Swedish Medical Products Agency, and registered on Eudra-CT (2020-001965-36)