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RNA extraction and RT-PCR for gene expression analysis To determine the effect of HT stress on the transcription levels of Calvin cycle enzyme genes and antioxidant enzyme genes and their changes after GSH application, we tested photosynthetic genes including those encoding ribulose-1,5-bisphosphate carboxylase/oxygenase activase ( RCA ), RBCL and RBCS involved in CO 2 fixation

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This reduces toxic load and supports liver function especially important for athletes using PEDs or those under pharmaceutical stress