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That means: 1 unit = 0.01 mL 10 units = 0.1 mL 50 units = 0.5 mL 100 units = 1.0 mL The "U-100" designation refers to the insulin concentration the syringe was designed for, not anything about peptides

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After washing of the trapping column with 0.1% trifluoroacetic acid (TFA), the peptides were eluted (flow rate300 nl/min) from the trapping column onto an analytical column (Aurora C18, 75 m ID, 250 mm long, 1.7 m particles, heated to 50 C, Ion Opticks) by 60 min linear gradient program (3-42 % of mobile phase B

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