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At the same time, the number of smokers increased about 7 percent to at least 869 million because of population growth, particularly in developing regions with relatively lax anti- smoking regulations, said Shane MacGuill, a Euromonitor tobacco analyst in London
After reverse transcription, the solution was diluted with 40 L of water and 2.5 L of the diluted cDNA solution was used for real-time PCR with the PowerUp SYBR mix and specific primers listed in Appendix Table S4
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