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Its always best practice to reconstitute and draw just before use

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Similar to the systemic calcium signal transmission upon GSH treatment, Glu-induced [Ca 2+ ] cyt propagation in systemic leaves were strongly inhibited in GCaMP3/glr3.3 while remained unaffected in GCaMP3/glr3.6 (Supplementary Fig

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This could indicate that MOR desensitization and tolerance are two unrelated mechanisms