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This alteration is associated with the synergistic effects of multiple active components in GQD, particularly alkaloids such as berberine and flavonoids such as baicalin, which have been reported to promote beneficial bacterial growth [38, 39]

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First, 0.15 g of EGCGZn nanocomposite powder was mixed with 0.3 g of pre-cooled glycerol (4 C) and ground at low temperature until a uniform paste was obtained