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Forgetting to swab a stopper, accidentally touching the needle to your finger, or working on a dirty surface can introduce bacteria

The resulting RNA was eluted with 45 l H 2 O and concentration as well as purity was measured with a NanoDrop ND-1000 spectrophotometer (NanoDrop, Wilmington, Delaware, USA)

The titre of the vector stocks was estimated to be 5.4 10 8 transducing units per ml for the Gsto2 -shRNA and 5.1 10 8 transducing units per ml for the scrambled shRNA

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