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Cells were washed with PBS and imaged in 11 mM glucose KRBH buffer using a Nikon Eclipse Ti microscope with an ORCA-Flash 4.0 camera (Hamamatsu) and Metamorph software (Molecular Devices) with a 20 air objective and 488 nm laser, with images taken every 6 seconds, on a 37 C stage

We tell you what types of foods to eat." If the patient is still hungry later, the next meal the plan is altered slightly, perhaps by including a higher quantity of healthy fats

Some light-sensitive peptides benefit from being wrapped in aluminum foil or stored in an opaque container