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Moreover, male mice allowed to the research group to adhere to 3 rs principles for animal studies, because the hyperglycemia threshold of 200 mg/dl fixed for the experimental plan and to proceed with semaglutide treatment was reached faster and more consistently (1011 weeks) with a less number of animals compared to female mice, which usually are protected against HFD-induced metabolic alterations [45], dilating time to achieve hyperglycemia condition

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The general idea around microdosing GLP-1s is that you intentionally take a reduced amount of a medication