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To maximize the capture of individual DUBs, we pooled probes from three different classes that included a mono-ubiquitin recognition element specific for DUBs, a biotin-tag for DUBs pulldown, and either a propargylamide (PA), a vinylmethylester (VME), or a vinylsulfone (VS) electrophilic warhead to covalently interact with the nucleophilic cysteine residue in the active sites of DUBs 15,16,17 (Fig

Latch struggles, soreness, and constantly questioning yourselfIs my baby getting enough?are incredibly common and can feel overwhelming at first

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