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The protein: inhibitor complex was purified by size-exclusion chromatography (Superdex Increase 75 pg 10/300 GL) in a final buffer containing 20 mM HEPES pH 7.5, 100 mM NaCl, 2 mM MgCl2, 5 mM TCEP, and 5 % glycerol to remove excess of nucleotides

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