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Melatonin protects mouse testes from palmitic acid-induced lipotoxicity by attenuating oxidative stress and DNA damage in a SIRT1-dependent manner

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The protein sample (250 g protein /30 L) was mixed with 270 L of UA (100 mM TrisHCl (pH 8.5) containing 8 M Urea) in the filter unit, and centrifuged at 14,000 g for 15 min