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The disease often leads to fetal losses and, in some cases, maternal mortality, generating significant economic consequences (Moghaddam and Hassanpour, 2008

PMID: 18763991

Many patients return periodically for maintenance treatments

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After incubation for 1 h at 20 C, 180 nL of glycerol (40% stock, final drop concentration 28.832.5%) were added to each drop immediately prior to crystal harvesting and flash-freezing in liquid nitrogen