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Homogenates were centrifuged at 10,000 g for 20 min at 4 C, and supernatants were collected and subjected to hydrophobic chromatography (C18 SepPak cartridges, Waters Corporation) according to the manufacturers instructions

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Treatment of obesity with semaglutide 2.4 mg in addition to D&E was cost-effective compared to D&E alone, with an ICER of 13,459 EUR per QALY gained, with incremental 1,325 EUR and 0.098 QALYs