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For a 75 to 90kg adult, that translates roughly to 190 to 900mcg daily, which brackets the 200 to 500mcg range being used in practice

10.1016/j.celrep.2015.04.064 Cell Rep

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[DOI] [PMC free article] [PubMed] [Google Scholar] Smits M.M., Tonneijck L., Muskiet M.H.A., Kramer M.H.H., Pieters-van den Bos I.C., Vendrik K.E.W., et al

Healthy follicles were identified by an intact basal lamina, a visible oocyte with a germinal vesicle, and a distinct nucleolus, while atretic follicles were characterized by pyknotic nuclei and degenerating granulosa cells.23 Immunohistochemistry The immunohistochemical staining kit (Thermo Ultravision Detection System, Fremont, USA, TP-125-HL) was used in conjunction with the streptavidin-biotin-peroxidase method