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Total RNA was extracted using the Total RNA Purification Kit and reverse-transcribed to cDNA using M-MLV reverse transcriptase

First, the allowable extent or concentration of denatured protein, peptide or antibody for a specific dosage must be identified analytically

High-dose N-acetylcysteine in stable COPD: the 1-year, double-blind, randomized, placebo-controlled HIACE study

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The organic layers from two extractions were combined and aliquots were analyzed on a 4000 Q-Trap mass spectrometer (AB SCIEX, Framingham, MA) using the 50 2.1 mm Hydrophilic Interaction Liquid Chromatography (HILIC-LC) column (Waters Corp., Milford, MA)