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T12M mean = 21.1 9.3 mol/L

The plate was then washed, followed by the addition of anti-human IgG Fc conjugated to HRP (Invitrogen) and incubation for 2 h at room temperature

J Biosci Bioeng 116(4):475479 Wang H (2013) Homocysteine induces inflammatory transcriptional signaling in monocytes

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A review study proposed another mechanisms underlying the potential interaction of the smoking and oestrogen metabolites such as decrease in oestrogen activity, SHBG levels, aromatase enzyme activity, C-20, 22 desmolase enzyme levels and increase in free testosterone levels, hyperandrogenism and the hepatic metabolism of steroid (Reference Ruan and Mueck41)