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The adverse effects associated with tirzepatide use in patients at increased risk of cardiovascular events: a systematic review with meta-analysis and Trial Sequential Analysis

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The rats received diluted AA (2 mL, 3% v/v) over 30 s using a 2-mm diameter polyurethane cannula placed 8 cm into the rats anus, and the volume and concentration of AA administered were standardized and reproducible to maintain consistency in the experimental protocol