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In the present study, it is noted that the expression of ferritin (FTH1), a protein functioning in maintaining iron homeostasis, was significantly decreased at the protein level, indicating that riboflavin deficiency can impact significantly on iron absorption and utilization 48,49

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For detection, 10 L of Reagent A and 20 L of Reagent B were sequentially added, and plates were incubated for 30 min at room temperature in the dark