Lv, Y., Liang, T., Wang, G., & Li, Z
instead, use smooth, slow swirling motions to transition the lyophilized pellet into solution without shearing the peptide chain
Lyophilized peptides must be stored at 20C, and once reconstituted with bacteriostatic water, refrigeration at 28C maintains stability for approximately 28 days
Reference standards typically appear as a fine lyophilized powder with a characteristic blue tint when adequate copper is bound visual confirmation, though not a substitute for HPLC and mass spectrometry
The percent (%) cytotoxicity was determined using the following formula for each given experimental treatment: Cytotoxicity (%) = 100 [(Experimental Culture Medium Background)/(Maximum LDH Release Culture Medium Background)]